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iDEC 2021 | OUC

CRISPR-guided DNA polymerases enable directed evolution of luciferase.

GraphicalOverview

EvolvR, a system that can continuously diversify all nucleotides within a tunable window length at user-defined site, is an attractive method for continuously directed evolution. Our project attempts to conduct directed evolution of luciferase lux of Marine Vibrio FG-1 isolated from the Yellow Sea by using EvolvR system, aiming to explore a luciferase mutant with better potential performance and various luminescence spectrum.

In addition, multiple gRNAs-guided EvolvR system is of great application value. However, due to the problem of genetic instability in the construction process of multiple gRNA, we propose the idea of using ELSA (Extra-long sgRNA Arrays) to help build multiple gRNAs-guided EvolvR system. We envision that a stable multiple gRNAs-guided EvolvR system will provide a new strategy for simultaneous evolution of multiple genes, such as metabolic pathways.

Our Team

Our team is based out of the College of Marine Life at Ocean University of China. Our team is made up of energetic undergraduate students who love life science.